Gene cloning is the production of a large number of copies of a gene by employing molecular biology techniques. The cloning is done by the introduction of a target gene into a plasmid which is a circular piece of DNA. The DNA is cut with the help of restriction enzyme and is joined with the plasmid with DNA ligase. This plasmid is then introduced into the bacterial cell by the process of transformation.
These bacterial cells are allowed to make more plasmid DNAs and to express the gene and form the final product that is a protein. This protein is then harvested from the bacterial cells and is purified. The clones of genes are also produced in labs by polymerase chain reaction (PCR).
How is an F′ factor different from an F factor?
Meiotic nondisjunction is much more likely than mitotic nondisjunction. Based on
In fruit flies, curved wings are recessive to straight wings,
What is an advantage and a disadvantage of this repair system?
Explain the type of speciation (allopatric, parapatric, or sympatric
The raw material for evolution is random mutation. Discuss whether or
With regard to the timing of conjugation, explain why the recipient
A woman who is heterozygous, Bb, has brown eyes;
A recessive allele in mice results in an unusally long neck.
Why is an antibody used in this experiment? From