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Question: Why is an antibody used in this

Why is an antibody used in this experiment? From figure 24.2:
Why is an antibody used in this experiment?

From figure 24.2:


Why is an antibody used in this experiment?

From figure 24.2:





Transcribed Image Text:

wwww -Protein of interest The goal is to determine where in the genome this protein binds. Add formaldehyde to crosslink protein to DNA. Lyse the cells. Sonicate DNA into small pieces Add antibodies that recognize the protein of interest. The antibodies are bound to heavy beads. After the antibodies bind to the protein of Interest, the sample is subjected to centrifugation. ww Protein of interestN bound to DNA and to an antibody Antibody against protein of interest - Pellet Вead Collect complexes in pellet. Add chemical that breaks the crossilinks to remove the protein. Conduct PCR using primers to a specific DNA region. Covalently attach DNA linkers to the ends of the DNA - Linker Conduct PCR using primers that are complementary to the linkers. Incorporate fluorescently labeled nucleotides during PCR. If PCR amplifies the DNA, the protein of interest was bound to the DNA region that is flanked by the primers. Denature DNA and hybridize to a microarray. See Figure 24.1. Fluorescently labeled spots identify sites in the genome where the protein of interest binds.


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